
Qualifying rained out: UPDATE: Persistant rain at Daytona has forced the cancellation of Nationwide Series qualifying. The field was set according to the rule book and #18-Kyle Busch will start on the pole, followed by #60-Carl Edwards, #88-Dale Earnhardt, Jr., #33-Kevin harvick, #21-Townley, #20-Logano, #32-Vickers, #38-Kahne, #16-Braun, and #6-Stenhouse. Did Not Qualify: #0-Clements, #35-Keller, #42-Kligerman, #49-Green, #52-Nuenberger, #56-Lepage, #70-Howard, #73-Cope, #75-Rowe, #83-Borneman, #92-Chapman, #96-Setzer, #98-Menard.
UPDATE: Some shuffling after qualifying was rained out: 5 cars (#97-Fuller, #52-Neuenberger, #96-Setzer, #49-Green, and #42-Kligerman) withdrew. #97-Fuller was the only one that had made the race. With his withdrawal, #98-Menard gets in.(2-12-2010)
| Pos. | Car # | Driver | Team | Reason |
| 1 | 18 | Kyle Busch | Interstate Toyota | 2009 Owners Points, 1st |
| 2 | 60 | Carl Edwards | Fastenal Ford | 2009 Owners Points, 2nd |
| 3 | 88 | Dale Earnhardt Jr. | Hellmann's Chevy | 2009 Owners Points, 3rd |
| 4 | 33 | Kevin Harvick | Jimmy John's Chevy | 2009 Owners Points, 4th |
| 5 | 21 | John Wes Townley | Zaxby's Chevy | 2009 Owners Points, 5th |
| 6 | 20 | Joey Logano | BioShock 2 / GameStop Toyota | 2009 Owners Points, 6th |
| 7 | 32 | Brian Vickers | Dollar General Toyota | 2009 Owners Points, 7th |
| 8 | 38 | Kasey Kahne | Great Clips Toyota | 2009 Owners Points, 8th |
| 9 | 16 | Colin Braun | Con-Way Ford | 2009 Owners Points, 9th |
| 10 | 6 | Ricky Stenhouse Jr. | Citi Ford | 2009 Owners Points, 10th |
| 11 | 1 | James Buescher | Phoenix Racing Chevy | 2009 Owners Points, 11th |
| 12 | 12 | Justin Allgaier | Verizon Wireless Dodge | 2009 Owners Points, 12th |
| 13 | 66 | Steve Wallace | 5-Hour Energy Toyota | 2009 Owners Points, 13th |
| 14 | 43 | Scott Lagasse Jr. | DrGMall.com Ford | 2009 Owners Points, 14th |
| 15 | 7 | Danica Patrick | GoDaddy.com Chevy | 2009 Owners Points, 15th |
| 16 | 62 | Brendan Gaughan | South Point Toyota | 2009 Owners Points, 16th |
| 17 | 99 | Trevor Bayne | Diamond-Waltrip Racing Toyota | 2009 Owners Points, 17th |
| 18 | 15 | Michael Annett | Pilot Travel Centers Toyota | 2009 Owners Points, 18th |
| 19 | 28 | Kenny Wallace | Blue Ox / Destination Daytona Chevy | 2009 Owners Points, 19th |
| 20 | 34 | Tony Raines | Long John Silver's Chevy | 2009 Owners Points, 20th |
| 21 | 40 | Mike Bliss | Key Motorsports Chevy | 2009 Owners Points, 21st |
| 22 | 10 | Jason Leffler | ABF Toyota | 2009 Owners Points, 22nd |
| 23 | 01 | Mike Wallace | Kendrick Meek Chevy | 2009 Owners Points, 23rd |
| 24 | 22 | Brad Keselowski | Discount Tire Dodge | 2009 Owners Points, 24th |
| 25 | 81 | Michael McDowell | D.H. Griffin Dodge | 2009 Owners Points, 25th |
| 26 | 24 | Eric McClure | Hefty Ford | 2009 Owners Points, 26th |
| 27 | 23 | Robert Richardson | Mahindra Tractors Chevy | 2009 Owners Points, 27th |
| 28 | 26 | Brian Keselowski | K-Automotive Motorsports Dodge | 2009 Owners Points, 28th |
| 29 | 61 | Josh Wise | Specialty Racing Ford | 2009 Owners Points, 29th |
| 30 | 87 | Joe Nemechek | D.A.B. Constructors Chevy | 2009 Owners Points, 30th |
| 31 | 27 | Greg Biffle | Red Man Ford | 2009 Winner [Las Vegas, Phoenix] |
| 32 | 4 | Tony Stewart | Oreo / Ritz Chevy | 2009 Winner [Daytona] |
| 33 | 05 | Jeff Green | Tobacco Free Florida / 31-W Chevy | Past Champion [2000] |
| 34 | 97 | Brad Teague | SponsorDavis.com Chevy | Qualifying Draw, 35th |
| 35 | 04 | Chrissy Wallace | Fuel-Doctor.com Chevy | Qualifying Draw, 36th |
| 36 | 41 | Danny Efland | CarLocate.com Chevy | Qualifying Draw, 37th |
| 37 | 02 | Brian Scott | StopRepairBills.com Toyota | Qualifying Draw, 38th |
| 38 | 11 | Johnny Satuer | GetMoreVacations.com Chevy | Qualifying Draw, 39th |
| 39 | 48 | Scott Riggs | RAB Racing Ford | Qualifying Draw, 41st |
| 40 | 09 | Morgan Shepherd | Fans for Faith Toyota | Qualifying Draw, 42nd |
| 41 | 89 | Stanton Barrett | Fuel Doctor FD-47 Chevy | Qualifying Draw, 43rd |
| 42 | 31 | Bobby Gerhardt | Lucas Oil Chevy | Qualifying Draw, 44th |
| 43 | 98 | Paul Menard | Menards Ford | Qualifying Draw, 49th |
