The fifth round of the 2021 eNASCAR iRacing Pro Invitational Series is set for Wednesday at virtual Chicago Street Course, with coverage beginning at 6 p.m. ET on FS1. The race, a 51-lapper featuring 37 cars and two resets, will get underway at 8 p.m. ET, also on FS1.
| No. | Team | Driver |
| 00 | StarCom Racing | Quin Houff |
| 2 | Team Penske | Brad Keselowski |
| 3 | Richard Childress Racing | Austin Dillon |
| 4 | Stewart-Haas Racing | Kevin Harvick |
| 5 | Hendrick Motorsports | Kyle Larson |
| 6 | Roush Fenway Racing | Ryan Newman |
| 7 | Spire Motorsports | Corey Lajoie |
| 8 | Richard Childress Racing | Tyler Reddick |
| 9 | Hendrick Motorsports | Chase Elliott |
| 10 | Stewart-Haas Racing | Aric Almirola |
| 11 | Joe Gibbs Racing | Denny Hamlin |
| 12 | Team Penske | Ryan Blaney |
| 14 | Stewart-Haas Racing | Chase Briscoe |
| 15 | Rick Ware Racing | James Davison |
| 17 | Roush Fenway Racing | Chris Buescher |
| 19 | Joe Gibbs Racing | Martin Truex, Jr. |
| 20 | Joe Gibbs Racing | Christopher Bell |
| 21 | Wood Brothers Racing | Matt DiBenedetto |
| 22 | Team Penske | Joey Logano |
| 23 | 23XI | Bubba Wallace |
| 24 | Hendrick Motorsports | William Byron |
| 38 | Front Row Motorsports | Anthony Alfredo |
| 41 | Stewart-Haas Racing | Cole Custer |
| 42 | Chip Ganassi Racing | Ross Chastain |
| 43 | Richard Petty Motorsports | Eric Jones |
| 47 | JTG Daugherty Racing | Ricky Stenhouse Jr. |
| 48 | Hendrick Motorsports | Alex Bowman |
| 51 | Petty Ware Racing | Garrett Smithley |
| 52 | Rick Ware Racing | Josh Bilicki |
| 53 | Rick Ware Racing | Joey Gase |
| 77 | Spire Motorsports | Justin Haley |
| 78 | Live Fast Motorsports | Kyle Tilley |
| 99 | Spire Motorsports | Daniel Suarez |
| 66 | MBM Motorsports | Timmy Hill |
| 88 | Promotor’s Provisional | Dale Earnhardt Jr. |
| 87 | Fan Vote | Jesse Iwuji |